Isolation of Polyhydroxybutyrate (PHB) Producing Bacteria, Optimization of Culture Conditions for PHB production, Extraction and Characterization of PHB
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چکیده
منابع مشابه
Cyanobacterial Polyhydroxybutyrate (PHB): Screening, Optimization and Characterization
In modern life petroleum-based plastic has become indispensable due to its frequent use as an easily available and a low cost packaging and moulding material. However, its rapidly growing use is causing aquatic and terrestrial pollution. Under these circumstances, research and development for biodegradable plastic (bioplastics) is inevitable. Polyhydroxybutyrate (PHB), a type of microbial polye...
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Polyhydroxybutyrate [P(3HB)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-CoA reductase (PhaB) and polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha (Cupriavidus necator) with optimized codon usage for expression in tobacco. P(3HB) contents in the transformants (0.2mg/g dry cell weight in average) harboring the codon-optimized phaB gene was twofold ...
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The undefined poly(3-hydroxybutyrate)- (PHB-) negative mutant R. eutropha PHB-4 was generated in 1970 by 1-nitroso-3-nitro-1-methylguanidine (NMG) treatment. Although being scientific relevant, its genotype remained unknown since its isolation except a recent first investigation. In this study, the mutation causing the PHA-negative phenotype of R. eutropha PHB-4 was confirmed independently: seq...
متن کاملLipases provide a new mechanistic model for polyhydroxybutyrate (PHB) synthases: characterization of the functional residues in Chromatium vinosum PHB synthase.
Polyhydroxybutyrate (PHB) synthases catalyze the conversion of beta-hydroxybutyryl coenzyme A (HBCoA) to PHB. These enzymes require an active site cysteine nucleophile for covalent catalysis. A protein BLASTp search using the Class III Chromatium vinosum synthase sequence reveals high homology to prokaryotic lipases whose crystal structures are known. The homology is very convincing in the alph...
متن کاملIsolation and Identification of Polyhydroxybutyrate(PHB) Producing Bacillus cereus BB613-A Novel Isolate
Poly-3-hydroxy butyrate (PHB) is a copolymer of polyhydroxyalkonate family. It accumulates in the intracellular granules of bacteria under nitrogen limiting environment. The PHB producing bacteria was isolated from farm soil by serial dilution method. The Sudan Black B staining and Nile Blue A staining were carried out to confirm that the isolated strain was capable of producing PHB. Further, i...
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ژورنال
عنوان ژورنال: Nepal Journal of Biotechnology
سال: 2019
ISSN: 2467-9313,2091-1130
DOI: 10.3126/njb.v6i1.22339